|Application ||WB, IHC-P, FC, E|
|Calculated MW||20478 Da|
|Antigen Region||157-185 aa|
|Other Names||Dual specificity protein phosphatase 3, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, VHR, DUSP3, VHR|
|Target/Specificity||This DUSP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-185 amino acids from the C-terminal region of human DUSP3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DUSP3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.|
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Provided below are standard protocols that you may find useful for product applications.
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. The gene for this protein maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer.
Alonso, A., et al., Nat. Immunol. 4(1):44-48 (2003).
Alonso, A., et al., J. Biol. Chem. 276(7):4766-4771 (2001).
Todd, J.L., et al., J. Biol. Chem. 274(19):13271-13280 (1999).
Kamb, A., et al., Genomics 23(1):163-167 (1994).
Folander, K., et al., Genomics 23(1):295-296 (1994).
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