|Application ||WB, IHC-P, E|
|Calculated MW||49831 Da|
|Antigen Region||11-40 aa|
|Other Names||Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, PPM1F, KIAA0015, POPX2|
|Target/Specificity||This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human PPM1F.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PPM1F Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dephosphorylates and concomitantly deactivates CaM- kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.|
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Provided below are standard protocols that you may find useful for product applications.
PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis.
Harvey, B.P., et al., J. Biol. Chem. 279(23):24889-24898 (2004).
Izmailova, E., et al., Nat. Med. 9(2):191-197 (2003).
Koh, C.G., et al., Curr. Biol. 12(4):317-321 (2002).
Tan, K.M., et al., J. Biol. Chem. 276(47):44193-44202 (2001).
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