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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PP2A alpha Antibody (C-term)   

PP2A alpha Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PP2A alpha Antibody (C-term) AP8462b
    All lanes : Anti-PPP2CA/B Antibody (F289) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - PP2A alpha Antibody (C-term) AP8462b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P67775
Other Accession P23696, P62716, P11611, P11493, P62715, P62714, Q0P594, P63331, P67777, P67776, P63330, P48463, P67774
Reactivity Human
Predicted Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35594 Da
Antigen Region 274-304 aa
Additional Information
Gene ID 5515
Other Names Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA
Target/Specificity This PP2A alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the C-terminal region of human PP2A alpha.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPP2A alpha Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Cellular Location Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity).
Research Areas
Citations (0)

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PPP2CA/B represents the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.


Gergs, U., et al., J. Biol. Chem. 279(39):40827-40834 (2004).
Prickett, T.D., et al., J. Biol. Chem. 279(37):38912-38920 (2004).
Scott, G.K., et al., EMBO J. 22(23):6234-6244 (2003).
Rao, R.K., et al., Biochem. Biophys. Res. Commun. 293(1):610-616 (2002).
Avdi, N.J., et al., J. Biol. Chem. 277(43):40687-40696 (2002).

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$ 295.00
$ 99.00
Cat# AP8462b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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