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GPD1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GPD1 Antibody (N-term) AP8507a
    Western blot analysis of lysate from human liver tissue lysate, using GPD1 Antibody (N-term)(Cat. #AP8507a). AP8507a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
    detail
  • IHC-P - GPD1 Antibody (N-term) AP8507a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with GPD1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GPD1 Antibody (N-term) AP8507a
    GPD1 Antibody (N-term) (Cat.#AP8507a) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P21695
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37568 Da
Antigen Region 28-57 aa
Additional Information
Gene ID 2819
Other Names Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, GPD-C, GPDH-C, GPD1
Target/Specificity This GPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human GPD1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPD1 (HGNC:4455)
Function Has glycerol-3-phosphate dehydrogenase activity.
Cellular Location Cytoplasm.
Tissue Location Expressed in liver (at protein level).
Research Areas
Citations (0)
citation

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Background

GRB14, GPD1, and GDF8 as potential network collaborators in weight loss-induced improvements in insulin action in human skeletal muscle.

References

Krasnov,G.S., et.al., Mol. Biol. (Mosk.) 43 (2), 348-356 (2009)
Park,J.J., et.al., Physiol. Genomics 27 (2), 114-121 (2006)

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$ 182.50
$ 70.00
Cat# AP8507a
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