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ACTN1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ACTN1 Antibody (N-term) AP8512a
    Western blot analysis of ACTN1 Antibody (N-term) (Cat. #AP8512a) in MDA-MB231 cell line lysates (35ug/lane). ACTN1 (arrow) was detected using the purified Pab.
  • IHC-P - ACTN1 Antibody (N-term) AP8512a
    Formalin-fixed and paraffin-embedded human breast carcinoma with ACTN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ACTN1 Antibody (N-term) AP8512a
    Flow cytometric analysis of MDA-231 cells using ACTN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12814
Other Accession Q9Z1P2, Q7TPR4, Q2PFV7, Q3B7N2
Reactivity Human
Predicted Bovine, Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 103058 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 87
Other Names Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1
Target/Specificity This ACTN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ACTN1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTN1
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Cellular Location Cytoplasm, cytoskeleton Cytoplasm, myofibril, sarcomere, Z line. Cell membrane. Cell junction. Cell projection, ruffle Note=Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures (By similarity).
Research Areas
Citations (0)

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ACTN1 is a F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.


Zhang,Z.,, J. Biol. Chem. 281 (3), 1746-1754 (2006)

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Cat# AP8512a
(40 western blots)
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