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LRPAP1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - LRPAP1 Antibody (C-term) AP8529b
    LRPAP1 Antibody (C-term) (Cat. #AP8529b) western blot analysis in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates the LRPAP1 antibody detected the LRPAP1 protein (arrow).
  • IHC-P - LRPAP1 Antibody (C-term) AP8529b
    Formalin-fixed and paraffin-embedded human brain tissue with LRPAP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - LRPAP1 Antibody (C-term) AP8529b
    Flow cytometric analysis of HepG2 cells using LRPAP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P30533
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41466 Da
Antigen Region 281-309 aa
Additional Information
Gene ID 4043
Other Names Alpha-2-macroglobulin receptor-associated protein, Alpha-2-MRAP, Low density lipoprotein receptor-related protein-associated protein 1, RAP, LRPAP1, A2MRAP
Target/Specificity This LRPAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-309 amino acids from the C-terminal region of human LRPAP1.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLRPAP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LRPAP1
Synonyms A2MRAP
Function Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.
Cellular Location Rough endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment lumen. Golgi apparatus, cis-Golgi network. Golgi apparatus lumen. Endosome lumen. Cell surface. Note=May be associated with receptors at the cell surface.
Research Areas
Citations ( 0 )

Background

LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

References

Lisi,S., et.al., J. Endocrinol. Invest. 30 (10), 839-843 (2007)
Vertegaal,A.C., et.al., Mol. Cell Proteomics 5 (12), 2298-2310 (2006)

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$ 295.00
$ 99.00
Cat# AP8529b
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(40 western blots)
Availability: In Stock
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