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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IL1B Antibody (Center)   

IL1B Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - IL1B Antibody (Center) AP8531C
    Overlay histogram showing MCF-7 cells stained with AP8531c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8531c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - IL1B Antibody (Center) AP8531C
    All lanes : Anti-IL1B Antibody (Center) at 1:2000 dilution Lane 1: human spleen lysate Lane 2: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IL1B Antibody (Center) AP8531C
    Western blot analysis of IL1B Antibody (Center) (Cat. #AP8531c) in NCI-H460 cell line lysates (35ug/lane). IL1B (arrow) was detected using the purified Pab.
  • IHC-P - IL1B Antibody (Center) AP8531C
    Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P01584
Other Accession P14628, P79182
Reactivity Human, Mouse
Predicted Monkey, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 30748 Da
Antigen Region 148-174 aa
Additional Information
Gene ID 3553
Other Names Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
Target/Specificity This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-174 amino acids of human IL1B.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL1B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL1B
Synonyms IL1F2
Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Cellular Location Cytoplasm, cytosol. Lysosome. Secreted, exosome {ECO:0000250|UniProtKB:P10749}. Cytoplasmic vesicle, autophagosome. Secreted Note=The precursor is cytosolic. In response to inflammasome- activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B- containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. {ECO:0000250|UniProtKB:P10749, ECO:0000269|PubMed:11728343, ECO:0000269|PubMed:15192144, ECO:0000305|PubMed:24201029}
Tissue Location Expressed in activated monocytes/macrophages (at protein level).
Research Areas
Citations ( 0 )

Background

IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.

References

Yu,J., et.al., Am. J. Gastroenterol. (2009)
Ito,A., et.al., J. Biol. Chem. 271 (25), 14657-14660 (1996)

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$ 295.00
$ 99.00
Cat# AP8531C
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(40 western blots)
Availability: In Stock
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