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ABI1 Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP8535a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP8535a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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ABI1 Antibody (N-term) - Product info | |
| Application | WB, IHC, FC, IF
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| Primary Accession | Q8IZP0 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 55081 Da |
ABI1 Antibody (N-term) - Additional info | |
| Gene ID 10006 | |
| Other Names ABI1; SSH3BP1; Abl interactor 1; Abelson interactor 1; Abl-binding protein 4; Eps8 SH3 domain-binding protein; Nap1-binding protein; Spectrin SH3 domain-binding protein 1; e3B1 | |
| Target/Specificity This ABI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87~117 amino acids from the N-terminal region of human ABI1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ABI1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ABI1 Antibody (N-term) - Protein Information | |
| Name ABI1 | |
| Synonyms SSH3BP1 | |
| Function May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level (By similarity) | |
| Cellular Location Cytoplasm (By similarity). Nucleus (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, filopodium (By similarity). Cell projection, growth cone (By similarity). Cell junction, synapse, synaptosome (By similarity). Cytoplasm, cytoskeleton (By similarity) Note=Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes (By similarity) | |
| Tissue Location Widely expressed, with highest expression in brain | |
ABI1 Antibody (N-term) - Related products
ABI1 Antibody (N-term) - Application data
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Western blot analysis of ABI1 Antibody (N-term) (Cat. #AP8535a) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
ABI1 Antibody (N-term) - Research Areas
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BACKGROUND
ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.
REFERENCES
Wang,C., et.al., Mol. Cancer Res. 5 (10), 1031-1039 (2007) Carabeo,R.A., et.al., Cell. Microbiol. 9 (9), 2278-2288 (2007)
