|Application ||WB, IHC-P, FC, IF, E|
|Other Accession||P62484, Q9NYB9, Q9QZM5, Q8CBW3, F1R187|
|Calculated MW||55081 Da|
|Antigen Region||81-108 aa|
|Other Names||Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1, ABI1, SSH3BP1|
|Target/Specificity||This ABI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human ABI1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ABI1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.|
|Cellular Location||Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, growth cone. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cytoplasm, cytoskeleton. Note=Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity).|
|Tissue Location||Widely expressed, with highest expression in brain.|
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Provided below are standard protocols that you may find useful for product applications.
ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.
Wang,C., et.al., Mol. Cancer Res. 5 (10), 1031-1039 (2007)
Carabeo,R.A., et.al., Cell. Microbiol. 9 (9), 2278-2288 (2007)
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