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ABI1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ABI1 Antibody (N-term) AP8535A
    Western blot analysis of ABI1 Antibody (N-term) (Cat. #AP8535a) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab.
  • IHC-P - ABI1 Antibody (N-term) AP8535A
    Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ABI1 Antibody (N-term) AP8535A
    Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - ABI1 Antibody (N-term) AP8535A
    Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8IZP0
Other Accession P62484, Q9NYB9, Q9QZM5, Q8CBW3, F1R187
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 55081 Da
Antigen Region 81-108 aa
Additional Information
Gene ID 10006
Other Names Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1, ABI1, SSH3BP1
Target/Specificity This ABI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human ABI1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABI1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABI1
Synonyms SSH3BP1
Function May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
Cellular Location Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, growth cone. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cytoplasm, cytoskeleton. Note=Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity).
Tissue Location Widely expressed, with highest expression in brain.
Research Areas
Citations (0)

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ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.


Wang,C.,, Mol. Cancer Res. 5 (10), 1031-1039 (2007)
Carabeo,R.A.,, Cell. Microbiol. 9 (9), 2278-2288 (2007)

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Cat# AP8535A
(40 western blots)
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