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AOC3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AOC3 Antibody (Center) AP8538c
    Western blot analysis of AOC3 Antibody (Center) (Cat. #AP8538c) in CEM cell line lysates (35ug/lane).AOC3 (arrow) was detected using the purified Pab.
    detail
  • WB - AOC3 Antibody (Center) AP8538c
    All lanes : Anti-AOC3 Antibody (Center) at 1:2000 dilution Lane 1: human lung lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - AOC3 Antibody (Center) AP8538c
    Formalin-fixed and paraffin-embedded human colon carcinoma with AOC3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • IHC-P - AOC3 Antibody (Center) AP8538c
    AP8538c staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - AOC3 Antibody (Center) AP8538c
    Flow cytometric analysis of CEM cells using AOC3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q16853
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 84622 Da
Antigen Region 613-640 aa
Additional Information
Gene ID 8639
Other Names Membrane primary amine oxidase, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, SSAO, Vascular adhesion protein 1, VAP-1, AOC3, VAP1
Target/Specificity This AOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-640 amino acids from the Central region of human AOC3.
Dilution WB~~1:2000
IHC-P~~1:25
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAOC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AOC3
Synonyms VAP1
Function Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.
Cellular Location Cell membrane; Single-pass type II membrane protein
Tissue Location Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. Isoform 2 seems to be the predominant transcript in fetal kidneys, fetal cartilage and fetal tonsils. The highest relative expression of isoform 2 occurs in skeletal muscle, heart, pancreas, kidney, and lung
Research Areas
Citations (0)
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Background

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity.

References

Lalor,P.F., et.al., J. Immunol. 169 (2), 983-992 (2002)
Salmi,M., et.al., Am. J. Pathol. 161 (6), 2255-2262 (2002)

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$ 365.00
$ 140.00
Cat# AP8538c
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