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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IFNB1 Antibody (N-term)   

IFNB1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - IFNB1 Antibody (N-term) AP8539A
    Overlay histogram showing HepG2 cells stained with AP8539a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8539a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - IFNB1 Antibody (N-term) AP8539A
    Anti-IFNB1 Antibody (N-term) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IFNB1 Antibody (N-term) AP8539A
    Western blot analysis of IFNB1 Antibody (N-term) (Cat. #AP8539a) in HepG2 cell line lysates (35ug/lane). IFNB1 (arrow) was detected using the purified Pab.
  • IHC-P - IFNB1 Antibody (N-term) AP8539A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with IFNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01574
Other Accession O77812
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22294 Da
Antigen Region 39-66 aa
Additional Information
Gene ID 3456
Other Names Interferon beta, IFN-beta, Fibroblast interferon, IFNB1, IFB, IFNB
Target/Specificity This IFNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human IFNB1.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIFNB1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFNB1
Synonyms IFB, IFNB
Function Has antiviral, antibacterial and anticancer activities.
Cellular Location Secreted.
Research Areas
Citations (0)

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IFNB1 has antiviral, antibacterial and anticancer activities.


Lienenklaus,S.,, J. Immunol. 183 (5), 3229-3236 (2009)
Eto,, Nat. Med. 5 (5), 577-581 (1999)

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$ 295.00
$ 99.00
Cat# AP8539A
(40 western blots)
Availability: In Stock
Bulk Size
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