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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CALM1 Antibody (C-term)   

CALM1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CALM1 Antibody (C-term) AP8549b
    Western blot analysis of CALM1 Antibody (C-term) (Cat. #AP8549b) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml)
  • FC - CALM1 Antibody (C-term) AP8549b
    CALM1 Antibody (C-term) (Cat.#AP8549b) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - CALM1 Antibody (C-term) AP8549b
    CALM1 Antibody (C-term) (Cat. #AP8549b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - CALM1 Antibody (C-term) AP8549b
    Confocal immunofluorescent analysis of CALM1 Antibody (C-term)(Cat. #AP8549b) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, IF, E
Primary Accession P62158
Other Accession P05419, P62155, P62161, P62160, P62204, P62152, Q6PI52, P62149, O16305, P62157, NP_008819, Q6YNX6
Reactivity Human
Predicted Bovine, C.Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Sheep, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16838 Da
Antigen Region 107-132 aa
Additional Information
Gene ID 801;805;808
Other Names Calmodulin, CaM, CALM1, CALM, CAM, CAM1
Target/Specificity This CALM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-132 amino acids from the C-terminal region of human CALM1.
Dilution WB~~1:1000
FC~~1:10~50
IHC-P~~1:50~100
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCALM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CALM1
Synonyms CALM, CAM, CAM1
Function Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Cellular Location Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules
Research Areas
Citations (0)

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Background

CALM1 is a member of the EF-hand calcium-binding protein family.Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

References

Zhao,D., et.al., Zhonghua Yi Xue Za Zhi 88 (35), 2452-2456 (2008)
Martins-de-Souza,D., et.al., J. Neural Transm. 116 (3), 275-289 (2009)

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$ 295.00
$ 99.00
Cat# AP8549b
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Quantity:
(40 western blots)
Availability: In Stock
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