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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PSMB9 Antibody (C-term)   

PSMB9 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PSMB9 Antibody (C-term) AP8556b
    All lanes : Anti-PSMB9 Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PSMB9 Antibody (C-term) AP8556b
    Western blot analysis of PSMB9 Antibody (C-term) (Cat. #AP8556b) in HL-60 cell line lysates (35ug/lane). PSMB9 (arrow) was detected using the purified Pab.
  • IHC-P - PSMB9 Antibody (C-term) AP8556b
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PSMB9 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PSMB9 Antibody (C-term) AP8556b
    PSMB9 Antibody (C-term) (Cat. #AP8556b) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P28065
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23264 Da
Antigen Region 193-219 aa
Additional Information
Gene ID 5698
Other Names Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein, PSMB9, LMP2, PSMB6i, RING12
Target/Specificity This PSMB9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-219 amino acids from the C-terminal region of human PSMB9.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB9
Synonyms LMP2, PSMB6i, RING12
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus
Research Areas
Citations (0)

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Background

PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

References

Honcharov,S.V., et.al., Fiziol Zh 55 (2), 3-10 (2009)
Moschonas,A., et.al., Mol. Cell. Biol. 28 (20), 6208-6222 (2008)

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$ 295.00
$ 99.00
Cat# AP8556b
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(40 western blots)
Availability: In Stock
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