- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
ADH5 Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP8562c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP8562c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ADH5 Antibody (Center) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P11766 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 39724 Da |
ADH5 Antibody (Center) - Additional info | |
| Gene ID 128 | |
| Other Names ADH5; ADHX; FDH; Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; S-(hydroxymethyl)glutathione dehydrogenase | |
| Target/Specificity This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219~248 amino acids from the Center region of human ADH5. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ADH5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ADH5 Antibody (Center) - Protein Information | |
| Name ADH5 | |
| Synonyms ADHX, FDH | |
| Function Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione | |
| Cellular Location Cytoplasm. | |
ADH5 Antibody (Center) - Related products
AP8562c: ADH5 Antibody (Center)
DC03926: Human ADH5 cDNA Clone
LY11041a: ADH5 Over-expression Lysate
ADH5 Antibody (Center) - Application data
-
Western blot analysis of ADH5 Antibody (Center) (Cat. #AP8562c) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab.
-
Western blot analysis of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody (Center) (Cat. #AP8562c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.
-
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Flow cytometric analysis of K562 cells using ADH5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ADH5 Antibody (Center) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.
REFERENCES
Martins-de-Souza,D., et.al., Eur Arch Psychiatry Clin Neurosci 259 (3), 151-163 (2009) Iborra,F.J., et.al., J. Histochem. Cytochem. 40 (12), 1865-1878 (1992)
