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ATP5O Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP5O Antibody (N-term) AP8563a
    Western blot analysis of ATP5O Antibody (N-term) (Cat. #AP8563a) in 293 cell line lysates (35ug/lane). ATP5O (arrow) was detected using the purified Pab.
  • IHC-P - ATP5O Antibody (N-term) AP8563a
    Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - ATP5O Antibody (N-term) AP8563a
    Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
  • FC - ATP5O Antibody (N-term) AP8563a
    Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - ATP5O Antibody (N-term) AP8563a
    Western blot analysis of ATP5O (arrow) using rabbit polyclonal ATP5O Antibody (N-term) (Cat. #AP8563a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATP5O gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48047
Other Accession Q2EN81, P13621
Reactivity Human
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23277 Da
Antigen Region 38-64 aa
Additional Information
Gene ID 539
Other Names ATP synthase subunit O, mitochondrial, Oligomycin sensitivity conferral protein, OSCP, ATP5O, ATPO
Target/Specificity This ATP5O antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-64 amino acids from the N-terminal region of human ATP5O.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP5O Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP5O
Synonyms ATPO
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Cellular Location Mitochondrion. Mitochondrion inner membrane
Research Areas
Citations (0)

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ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.


Wang,L.,, Cancer Epidemiol. Biomarkers Prev. 17 (12), 3558-3566 (2008)
Contessi,S.,, J. Bioenerg. Biomembr. 39 (4), 291-300 (2007)

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$ 295.00
$ 99.00
Cat# AP8563a
(40 western blots)
Availability: In Stock
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