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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SCAP Antibody (Center)   

SCAP Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SCAP Antibody (Center) AP8568c
    All lanes : Anti-SCAP Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: LNCap whole cell lysate Lane 5: Mouse brain lysate Lane 6: Mouse liver lysate Lane 7: Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 140, 98, 96 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SCAP Antibody (Center) AP8568c
    All lanes : Anti-SCAP Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: LNCap whole cell lysate Lane 5: Mouse brain lysate Lane 6: Mouse liver lysate Lane 7: Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 140, 98, 96 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SCAP Antibody (Center) AP8568c
    Western blot analysis of lysate from PC-3 cell line, using SCAP Antibody (Center)(Cat. #AP8568c). AP8568c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
  • IHC-P - SCAP Antibody (Center) AP8568c
    Formalin-fixed and paraffin-embedded human colon carcinoma with SCAP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SCAP Antibody (Center) AP8568c
    Flow cytometric analysis of K562 cells using SCAP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - SCAP Antibody (Center) AP8568c
    Fluorescent confocal image of HeLa cells stained with SCAP (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8568c SCAP (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession Q12770
Other Accession A2RRU4, Q5MNU5, Q6GQT6, A6QM06
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 139729 Da
Antigen Region 604-632 aa
Additional Information
Gene ID 22937
Other Names Sterol regulatory element-binding protein cleavage-activating protein, SCAP, SREBP cleavage-activating protein, SCAP, KIAA0199
Target/Specificity This SCAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-632 amino acids from the Central region of human SCAP.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCAP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SCAP
Synonyms KIAA0199
Function Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Research Areas
Citations ( 0 )

Background

SCAP is a protein with a sterol sensing domain(SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis.

References

Lu,Y., et.al., J. Lipid Res. 49 (12), 2582-2589 (2008)

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$ 295.00
$ 99.00
Cat# AP8568c
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(40 western blots)
Availability: in stock
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