|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8R5K6, Q61471|
|Calculated MW||38155 Da|
|Antigen Region||54-83 aa|
|Other Names||Protein Tob1, Transducer of erbB-2 1, TOB1, TOB, TROB1|
|Target/Specificity||This TOB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human TOB1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TOB1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex (PubMed:8632892, PubMed:23236473). Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay (PubMed:21336257).|
|Cellular Location||Cytoplasm. Nucleus. Note=Only a small fraction localizes to the cytoplasm except in late S-phase where more than half of proteins become cytoplasmic.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
TOB1 is a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.
Suzuki,T., et.al., Genes Dev. 16 (11), 1356-1370 (2002)
Yoshida,Y., et.al., Cell 103 (7), 1085-1097 (2000)
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