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>   home   >   Products   >   Primary Antibodies   >   HSPC142 Antibody (Center)   

HSPC142 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - HSPC142 Antibody (Center) AP8573c
    Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - HSPC142 Antibody (Center) AP8573c
    Western blot analysis of HSPC142 Antibody (Center) (Cat.#AP8573c) in MDA-MB231 cell line lysates (35ug/lane). HSPC142 (arrow) was detected using the purified Pab.
  • FC - HSPC142 Antibody (Center) AP8573c
    Flow cytometric analysis of MDA-MB468 cells using HSPC142 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, E
Primary Accession Q9NWV8
Other Accession Q5XIJ6, Q3UI43, Q08E57
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36560 Da
Antigen Region 116-143 aa
Additional Information
Gene ID 29086
Other Names BRISC and BRCA1-A complex member 1, Mediator of RAP80 interactions and targeting subunit of 40 kDa, New component of the BRCA1-A complex, BABAM1, C19orf62, MERIT40, NBA1
Target/Specificity This HSPC142 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human HSPC142.
Dilution IHC-P~~1:50~100
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPC142 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BABAM1
Function Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys- 63'-linked ubiquitin in various substrates (PubMed:24075985, PubMed:26195665). In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
Cellular Location Cytoplasm. Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs)
Citations (0)

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Background

Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.

References

Shao,G., et.al., Genes Dev. 23 (6), 740-754 (2009)
Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)

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Cat# AP8573c
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(40 western blots)
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