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C5 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - C5 Antibody (N-term) AP8577a
    Western blot analysis of C5 Antibody (N-term) (Cat. #AP8577a) in HL-60 cell line lysates (35ug/lane). C5 (arrow) was detected using the purified Pab.(2ug/ml)
  • IHC-P - C5 Antibody (N-term) AP8577a
    Formalin-fixed and paraffin-embedded human breast carcinoma with C5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - C5 Antibody (N-term) AP8577a
    Flow cytometric analysis of MDA-231 cells using C5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01031
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 188305 Da
Antigen Region 253-281 aa
Additional Information
Gene ID 727
Other Names Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5 beta chain, Complement C5 alpha chain, C5a anaphylatoxin, Complement C5 alpha' chain, C5, CPAMD4
Target/Specificity This C5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the N-terminal region of human C5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name C5
Synonyms CPAMD4
Function Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Cellular Location Secreted.
Research Areas
Citations (0)

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C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.


McClure,A.,, Rheumatology (Oxford) 48 (11), 1369-1374 (2009)
DiScipio,, J. Biol. Chem. 267 (24), 17087-17094 (1992)

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$ 295.00
$ 99.00
Cat# AP8577a
(40 western blots)
Availability: In Stock
Bulk Size
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