|Application ||WB, IHC-P, FC, E|
|Calculated MW||188305 Da|
|Antigen Region||253-281 aa|
|Other Names||Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5 beta chain, Complement C5 alpha chain, C5a anaphylatoxin, Complement C5 alpha' chain, C5, CPAMD4|
|Target/Specificity||This C5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the N-terminal region of human C5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||C5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.|
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Provided below are standard protocols that you may find useful for product applications.
C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.
McClure,A., et.al., Rheumatology (Oxford) 48 (11), 1369-1374 (2009)
DiScipio,R.G.et.al., J. Biol. Chem. 267 (24), 17087-17094 (1992)
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