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ACSS3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ACSS3 Antibody (Center) AP8579c
    Anti-ACSS3 Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ACSS3 Antibody (Center) AP8579c
    Western blot analysis of ACSS3 Antibody (Center) (Cat. #AP8579c) in NCI-H460 cell line lysates (35ug/lane). ACSS3 (arrow) was detected using the purified Pab.(2ug/ml)
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H6R3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 74778 Da
Antigen Region 224-251 aa
Additional Information
Gene ID 79611
Other Names Acyl-CoA synthetase short-chain family member 3, mitochondrial, Acyl-CoA synthetase short-chain family member 3, ACSS3
Target/Specificity This ACSS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the Central region of human ACSS3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACSS3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACSS3
Function Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:28003429). Propionate is the preferred substrate (PubMed:28003429). Can utilize acetate and butyrate with a much lower affinity (By similarity).
Cellular Location Mitochondrion matrix
Research Areas
Citations (0)
citation

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Background

ACSS3 activates acetate so that it can be used for lipid synthesis or for energy generation.

References

Watkins,P.A., Maiguel,D., et.al., J. Lipid Res. 48 (12), 2736-2750 (2007)

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$ 365.00
$ 140.00
Cat# AP8579c
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