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RARS2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RARS2 Antibody (C-term) AP8590b
    Anti-RARS2 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - RARS2 Antibody (C-term) AP8590b
    Western blot analysis of RARS2 Antibody (C-term) (Cat. #AP8590b) in HepG2 cell line lysates (35ug/lane). RARS2 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - RARS2 Antibody (C-term) AP8590b
    Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with RARS2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - RARS2 Antibody (C-term) AP8590b
    Flow cytometric analysis of NCI-H292 cells using RARS2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q5T160
Other Accession Q0P5H7
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 65505 Da
Antigen Region 369-399 aa
Additional Information
Gene ID 57038
Other Names Probable arginine--tRNA ligase, mitochondrial, Arginyl-tRNA synthetase, ArgRS, RARS2, RARSL
Target/Specificity This RARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-399 amino acids from the C-terminal region of human RARS2.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRARS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RARS2
Synonyms RARSL
Function Catalyzes the attachment of arginine to tRNA(Arg) in a two- step reaction: arginine is first activated by ATP to form Arg-AMP and then transferred to the acceptor end of tRNA(Arg).
Cellular Location Mitochondrion membrane
Research Areas
Citations (0)
citation

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Background

RARS2 is an arginyl-tRNA synthetase that is found in the mitochondrial matrix.

References

Edvardson,S., et.al., Am. J. Hum. Genet. 81 (4), 857-862 (2007)
Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)
Bonnefond,L., et.al., Biochemistry 44 (12), 4805-4816 (2005)

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$ 365.00
$ 140.00
Cat# AP8590b
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