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LMOD1 Antibody (Center N201)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LMOD1 Antibody (Center N201) AP8611c
    Western blot analysis of LMOD1 Antibody (Center N201) (Cat. #AP8611c) in Hela cell line lysates (35ug/lane). LMOD1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - LMOD1 Antibody (Center N201) AP8611c
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with LMOD1 Antibody (Center N201), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - LMOD1 Antibody (Center N201) AP8611c
    Flow cytometric analysis of hela cells using LMOD1 Antibody (Center N201)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P29536
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 67030 Da
Antigen Region 214-241 aa
Additional Information
Gene ID 25802
Other Names Leiomodin-1, 64 kDa autoantigen 1D, 64 kDa autoantigen 1D3, 64 kDa autoantigen D1, Leiomodin, muscle form, Smooth muscle leiomodin, SM-Lmod, Thyroid-associated ophthalmopathy autoantigen, LMOD1
Target/Specificity This LMOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-241 amino acids from the Central region of human LMOD1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLMOD1 Antibody (Center N201) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LMOD1
Function Required for proper contractility of visceral smooth muscle cells (PubMed:28292896). Mediates nucleation of actin filaments.
Cellular Location Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:A0A0G2K0D3}. Note=Colocalizes with actin filaments in sarcomeres. {ECO:0000250|UniProtKB:A0A0G2K0D3}
Tissue Location Detected in lung vascular smooth muscle (at protein level) (PubMed:27144530). Detected in thyroid and extraocular smooth muscle, but not skeletal muscle (PubMed:2026759). Detected in heart, aorta, skeletal muscle, colon, urinary bladder, uterus, stomach, and small intestine (PubMed:11318603).
Research Areas
Citations (0)
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Background

The leiomodin 1 protein has a putative membrane-spanning region and 2 types of tandemly repeated blocks.

References

Zhang,Z.G., et.al., Clin. Immunol. Immunopathol. 80 (3 PT 1), 236-244 (1996)
Conley,C.A.et.al., Curr. Eye Res. 19 (4), 313-322 (1999)

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$ 182.50
$ 70.00
Cat# AP8611c
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