Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Immunology   >   HLA-B Antibody (N-term)   

HLA-B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
Products labeled with a "crown" meet aggressive quality standards. Learn more.
  • FC - HLA-B Antibody (N-term) AP8616a
    Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AP8616a) compared to an isotype control of rabbit IgG (blue). AP8616a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • FC - HLA-B Antibody (N-term) AP8616a
    Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AP8616a) compared to an isotype control of rabbit IgG (blue). AP8616a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - HLA-B Antibody (N-term) AP8616a
    Western blot analysis of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(Cat. #AP8616a). AP8616a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - HLA-B Antibody (N-term) AP8616a
    Western blot analysis of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(Cat. #AP8616a). AP8616a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - HLA-B Antibody (N-term) AP8616a
    Western blot analysis of HLA-B Antibody (N-term) (Cat. #AP8616a) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
  • WB - HLA-B Antibody (N-term) AP8616a
    Western blot analysis of HLA-B Antibody (N-term) (Cat. #AP8616a) in A375 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
  • WB - HLA-B Antibody (N-term) AP8616a
    Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) (Cat. #AP8616a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.
  • IHC-P - HLA-B Antibody (N-term) AP8616a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - HLA-B Antibody (N-term) AP8616a
    HLA-B Antibody (N-term) (Cat.#AP8616a) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P03989
Other Accession P30490, P30488, P30487, P30486, P30485, P30483, P30481, P30479, Q04826, P18463, P30464, P30461
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40428 Da
Antigen Region 62-90 aa
Additional Information
Other Names HLA class I histocompatibility antigen, B-27 alpha chain, MHC class I antigen B*27, HLA-B, HLAB
Target/Specificity This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Dilution FC~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-B
Synonyms HLAB
Function Involved in the presentation of foreign antigens to the immune system.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

Background

HLA-B is involved in the presentation of foreign antigens to the immune system.

References

Higgins,C.M., et.al., Ann. Rheum. Dis. 51 (7), 855-862 (1992)
Guild,B.C.et.al., J. Biol. Chem. 259 (14), 9235-9240 (1984)
Orr,H.T., et.alt., Biochemistry 18 (25), 5711-5720 (1979)

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

$ 295.00
$ 99.00
Cat# AP8616a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
30% off on 400+ Stem Cells Antibodies. PromoCode: <span class=text-red>FLASH19</span>
Terms & Conditions