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TSHB Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - TSHB Antibody (Center) AP8617c
    Confocal immunofluorescent analysis of TSHB Antibody (Center)(Cat#AP8617c) with MDA-MB231 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - TSHB Antibody (Center) AP8617c
    Anti-TSHB Antibody (Center) at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - TSHB Antibody (Center) AP8617c
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - TSHB Antibody (Center) AP8617c
    TSHB Antibody (Center) (Cat. #AP8617c) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, IHC-P, E
Primary Accession P01222
Other Accession P12656
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 15639 Da
Antigen Region 58-86 aa
Additional Information
Gene ID 7252
Other Names Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, TSH-B, TSH-beta, Thyrotropin beta chain, Thyrotropin alfa, TSHB
Target/Specificity This TSHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human TSHB.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSHB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSHB
Function Indispensable for the control of thyroid structure and metabolism.
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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Background

TSHB is indispensable for the control of thyroid structure and metabolism.

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$ 365.00
$ 140.00
Cat# AP8617c
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