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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PRODH Antibody (Center)   

PRODH Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - PRODH Antibody (Center) AP8620c
    Overlay histogram showing A549 cells stained with AP8620c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8620c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - PRODH Antibody (Center) AP8620c
    AP8620c staining PRODH in H. skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - PRODH Antibody (Center) AP8620c
    Anti-PRODH Antibody (Center) at 1:2000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PRODH Antibody (Center) AP8620c
    Anti-PRODH Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PRODH Antibody (Center) AP8620c
    All lanes : Anti-PRODH Antibody (Center) at 1:1000 dilution Lane 1: MDA-MB-468 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PRODH Antibody (Center) AP8620c
    Western blot analysis of mouse Prodh Antibody (Center) (Cat. #AP8620c) in mouse liver, kidney tissue lysates (35ug/lane). PRODH (arrow) was detected using the purified Pab.
  • IHC-P - PRODH Antibody (Center) AP8620c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9WU79
Other Accession O43272
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68036 Da
Antigen Region 130-155 aa
Additional Information
Gene ID 19125
Other Names Proline dehydrogenase 1, mitochondrial, Proline oxidase, Prodh, Pro1
Target/Specificity This PRODH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-155 amino acids from the Central region of human PRODH.
Dilution FC~~1:25
IHC-P~~1:50~100
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRODH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Prodh
Synonyms Pro1
Function Converts proline to delta-1-pyrroline-5-carboxylate.
Cellular Location Mitochondrion matrix.
Tissue Location Expressed in liver, kidney, heart and to a lesser extent in brain, lung and muscle
Research Areas
Citations (0)

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Background

PRODH is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. It converts proline to delta-1-pyrroline-5-carboxylate.

References

Polyak,K., et.al., Nature 389 (6648), 300-305 (1997) Gogos,J.A., et.al., Nat. Genet. 21 (4), 434-439 (1999)

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$ 295.00
$ 99.00
Cat# AP8620c
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(40 western blots)
Availability: In Stock
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