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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ESRRA Antibody (Center)   

ESRRA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ESRRA Antibody (Center) AP8635c
    Western blot analysis of ESRRA Antibody (Center) (Cat. #AP8635c) in A375 cell line lysates (35ug/lane). ESRRA (arrow) was detected using the purified Pab.
  • IHC-P - ESRRA Antibody (Center) AP8635c
    Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ESRRA Antibody (Center) AP8635c
    Flow cytometric analysis of 293 cells using ESRRA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - ESRRA Antibody (Center) AP8635c
    Western blot analysis of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody (Center) (Cat. #AP8635c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.
  • IF - ESRRA Antibody (Center) AP8635c
    Confocal immunofluorescent analysis of ESRRA Antibody (Center) (Cat. #AP8635c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession P11474
Other Accession P62510, P62509, P62508, P11475, Q61539, O95718, Q5QJV7, O08580
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45510 Da
Antigen Region 131-159 aa
Additional Information
Gene ID 2101
Other Names Steroid hormone receptor ERR1, Estrogen receptor-like 1, Estrogen-related receptor alpha, ERR-alpha, Nuclear receptor subfamily 3 group B member 1, ESRRA, ERR1, ESRL1, NR3B1
Target/Specificity This ESRRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human ESRRA.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsESRRA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ESRRA
Synonyms ERR1, ESRL1, NR3B1
Function Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:18063693}
Research Areas
Citations (0)

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Background

ESRRA is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes.

References

Yang,N., et.al., J. Biol. Chem. 271 (10), 5795-5804 (1996)
Wiley,S.R., et.al., Genes Dev. 7 (11), 2206-2219 (1993)

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Cat# AP8635c
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(40 western blots)
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