|Application ||WB, IHC-P, FC, E|
|Calculated MW||33300 Da|
|Antigen Region||125-151 aa|
|Other Names||Max-like protein X, Class D basic helix-loop-helix protein 13, bHLHd13, Max-like bHLHZip protein, Protein BigMax, Transcription factor-like protein 4, MLX, BHLHD13, TCFL4|
|Target/Specificity||This MLX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human MLX.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MLX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.|
|Cellular Location||Isoform Alpha: Cytoplasm. Note=Found predominantly in the cytoplasm (PubMed:10918583). Isoform Gamma: Nucleus. Note=Found predominantly in the nucleus (PubMed:10918583).|
|Tissue Location||Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung|
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Provided below are standard protocols that you may find useful for product applications.
MLX belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This protein may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4.
Meroni,G., et.al., Oncogene 19 (29), 3266-3277 (2000)
Billin,A.N., et.al., J. Biol. Chem. 274 (51), 36344-36350 (1999)
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