|Application ||WB, IHC-P, E|
|Calculated MW||58875 Da|
|Antigen Region||466-494 aa|
|Other Names||25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 24-OHase, Vitamin D(3) 24-hydroxylase, Cytochrome P450 24A1, Cytochrome P450-CC24, CYP24A1, CYP24|
|Target/Specificity||This CYP24A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 466-494 amino acids from the C-terminal region of human CYP24A1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CYP24A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.|
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Provided below are standard protocols that you may find useful for product applications.
CYP24A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system.
Okuda,K., et.al., J. Lipid Res. 36 (8), 1641-1652 (1995)
Bosse,Y., et.al., Respir. Res. 10, 98 (2009)
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