|Application ||FC, WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||23490 Da|
|Antigen Region||176-204 aa|
|Other Names||Ras-related protein Rab-7a, RAB7A, RAB7|
|Target/Specificity||This RAB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human RAB7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAB7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.|
|Cellular Location||Late endosome. Lysosome. Cytoplasmic vesicle, phagosome. Melanosome. Cytoplasmic vesicle, phagosome membrane; Lipid- anchor; Cytoplasmic side. Note=Colocalizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity).|
|Tissue Location||Widely expressed; high expression found in skeletal muscle.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori.
Davies,J.P., et.al., Genomics 41 (1), 131-134 (1997)
Vitelli,R., et.al., Biochem. Biophys. Res. Commun. 229 (3), 887-890 (1996)
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