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RAB7 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - RAB7 Antibody (C-term) AP8665b
    AP8665b staining RAB7 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - RAB7 Antibody (C-term) AP8665b
    AP8665b staining RAB7 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - RAB7 Antibody (C-term) AP8665b
    AP8665b staining RAB7 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • FC - RAB7 Antibody (C-term) AP8665b
    Overlay histogram showing HepG2 cells stained with AP8665b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8665b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RAB7 Antibody (C-term) AP8665b
    All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RAB7 Antibody (C-term) AP8665b
    All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RAB7 Antibody (C-term) AP8665b
    All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession P51149
Other Accession P51150
Reactivity Human, Mouse, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23490 Da
Antigen Region 176-204 aa
Additional Information
Gene ID 7879
Other Names Ras-related protein Rab-7a, RAB7A, RAB7
Target/Specificity This RAB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human RAB7.
Dilution IHC-P~~1:25
FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB7A
Synonyms RAB7
Function Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF- EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Cellular Location Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein; Cytoplasmic side. Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Melanosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet {ECO:0000250|UniProtKB:P51150}. Endosome membrane. Note=Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255211). Lipid droplet localization is increased upon ADRB2 stimulation (By similarity) {ECO:0000250|UniProtKB:P51150, ECO:0000269|PubMed:16176980, ECO:0000269|PubMed:21255211}
Tissue Location Widely expressed; high expression found in skeletal muscle.
Research Areas
Citations (0)

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Background

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori.

References

Davies,J.P., et.al., Genomics 41 (1), 131-134 (1997)
Vitelli,R., et.al., Biochem. Biophys. Res. Commun. 229 (3), 887-890 (1996)

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$ 295.00
$ 99.00
Cat# AP8665b
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(40 western blots)
Availability: In Stock
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