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>   home   >   Products   >   Primary Antibodies   >   ALKBH3 Antibody (C-term)   

ALKBH3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ALKBH3 Antibody (C-term) AP8682b
    Western blot analysis of ALKBH3 Antibody (C-term) (Cat. #AP8682b) in mouse kidney tissue lysates (35ug/lane). ALKBH3 (arrow) was detected using the purified Pab.
  • IHC-P - ALKBH3 Antibody (C-term) AP8682b
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ALKBH3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96Q83
Other Accession Q32L00
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33375 Da
Antigen Region 242-269 aa
Additional Information
Gene ID 221120
Other Names Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, 11411-, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, ALKBH3, ABH3, DEPC1
Target/Specificity This ALKBH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human ALKBH3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALKBH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ABH3, DEPC1
Function Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
Cellular Location Cytoplasm. Nucleus.
Tissue Location Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon
Citations (0)

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The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.


Sundheim,O.,, EMBO J. 25 (14), 3389-3397 (2006)

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$ 295.00
$ 99.00
Cat# AP8682b
(40 western blots)
Availability: In Stock
Bulk Size
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