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ATP12A Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP12A Antibody (Center) AP8687c
    Western blot analysis of ATP12A Antibody (Center) (Cat. #AP8687c) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.
  • IHC-P - ATP12A Antibody (Center) AP8687c
    Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ATP12A Antibody (Center) AP8687c
    ATP12A Antibody (Center)(Cat. #AP8687c) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54707
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 115511 Da
Antigen Region 833-859 aa
Additional Information
Gene ID 479
Other Names Potassium-transporting ATPase alpha chain 2, Non-gastric H(+)/K(+) ATPase subunit alpha, Proton pump, ATP12A, ATP1AL1
Target/Specificity This ATP12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 833-859 amino acids from the Central region of human ATP12A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP12A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP12A
Synonyms ATP1AL1
Function Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues.
Cellular Location Membrane; Multi-pass membrane protein.
Tissue Location Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level)
Research Areas
Citations (0)

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ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues.


Li,J.,, Kidney Int. 65 (4), 1301-1310 (2004)

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$ 295.00
$ 99.00
Cat# AP8687c
(40 western blots)
Availability: In Stock
Bulk Size
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