|Application ||WB, IHC-P, FC, E|
|Other Accession||Q569A2, Q8BKT6, Q29RH7|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||35383 Da|
|Antigen Region||232-259 aa|
|Other Names||Tetraspanin-12, Tspan-12, Tetraspan NET-2, Transmembrane 4 superfamily member 12, TSPAN12, NET2, TM4SF12|
|Target/Specificity||This TSPAN12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-259 amino acids from the C-terminal region of human TSPAN12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TSPAN12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
Xu,D., et.al., FASEB J. 23 (11), 3674-3681 (2009)
Berditchevski,F.et.al., J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)
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