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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   TSPAN12 Antibody (C-term)   

TSPAN12 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TSPAN12 Antibody (C-term) AP8693b
    Western blot analysis of TSPAN12 Antibody (C-term) (Cat. #AP8693b) in T47D cell line lysates (35ug/lane). TSPAN12 (arrow) was detected using the purified Pab.
  • IHC-P - TSPAN12 Antibody (C-term) AP8693b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - TSPAN12 Antibody (C-term) AP8693b
    TSPAN12 Antibody (C-term) (Cat. #AP8693b) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95859
Other Accession Q569A2, Q8BKT6, Q29RH7
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35383 Da
Antigen Region 232-259 aa
Additional Information
Gene ID 23554
Other Names Tetraspanin-12, Tspan-12, Tetraspan NET-2, Transmembrane 4 superfamily member 12, TSPAN12, NET2, TM4SF12
Target/Specificity This TSPAN12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-259 amino acids from the C-terminal region of human TSPAN12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSPAN12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSPAN12
Synonyms NET2, TM4SF12
Function Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity.
Cellular Location Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)

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TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.


Xu,D.,, FASEB J. 23 (11), 3674-3681 (2009)
Berditchevski,, J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)

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$ 295.00
$ 99.00
Cat# AP8693b
(40 western blots)
Availability: In Stock
Bulk Size
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