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VIME Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - VIME Antibody AP8694a
    Western blot analysis of VIME Antibody (Cat. #AP8694a) in A375 cell line lysates (35ug/lane). VIME (arrow) was detected using the purified Pab.
  • IHC-P - VIME Antibody AP8694a
    VIME Antibody (Cat. #AP8694a)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VIME Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - VIME Antibody AP8694a
    VIME Antibody (Cat. #AP8694a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53652 Da
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity This Vimentin antibody is generated from rabbits immunized with Vimentin recombinant protein.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVIME Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations (0)

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VIME is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. This protein is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling.


Morishima,N., Genes Cells 4 (7), 401-414 (1999)

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$ 295.00
$ 99.00
Cat# AP8694a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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