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SREBF1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SREBF1 Antibody (Center) AP8732c
    Anti-SREBF1 Antibody (Center) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 122 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SREBF1 Antibody (Center) AP8732c
    Western blot analysis of SREBF1 Antibody (Center) (Cat. #AP8732c) in MDA-MB435 cell line lysates (35ug/lane). SREBF1 (arrow) was detected using the purified Pab.
  • IHC-P - SREBF1 Antibody (Center) AP8732c
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SREBF1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SREBF1 Antibody (Center) AP8732c
    SREBF1 Antibody (Center) (Cat. #AP8732c) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P36956
Other Accession P56720, Q9WTN3, Q60416
Reactivity Human
Predicted Hamster, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 121675 Da
Antigen Region 349-378 aa
Additional Information
Gene ID 6720
Other Names Sterol regulatory element-binding protein 1, SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1, Processed sterol regulatory element-binding protein 1, SREBF1, BHLHD1, SREBP1
Target/Specificity This SREBF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-378 amino acids from the Central region of human SREBF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSREBF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BHLHD1, SREBP1
Function Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols Isoform SREBP-1aDelta: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:18267114}
Tissue Location Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle
Research Areas
Citations (0)

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SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.


Wang,X.,, Cell 77 (1), 53-62 (1994)

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$ 295.00
$ 99.00
Cat# AP8732c
(40 western blots)
Availability: In Stock
Bulk Size
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