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SOD1 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP8733c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP8733c-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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SOD1 Antibody (Center) - Product info | |
| Application | WB, IHC, FC, IF
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| Primary Accession | P00441 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 15936 Da |
SOD1 Antibody (Center) - Additional info | |
| Gene ID 6647 | |
| Other Names SOD1; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1 | |
| Target/Specificity This SOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the Center region of human SOD1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SOD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SOD1 Antibody (Center) - Protein Information | |
| Name SOD1 | |
| Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems | |
| Cellular Location Cytoplasm. Note=The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria | |
SOD1 Antibody (Center) - Related products
AP8733c: SOD1 Antibody (Center)
DC00478: Human SOD1 cDNA Clone
LY11098a: SOD1 Over-expression Lysate
SOD1 Antibody (Center) - Application data
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Western blot analysis of SOD1 Antibody (Center) (Cat. #AP8733c) in Jurkat cell line lysates (35ug/lane). SOD1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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SOD1 Antibody (Center) (Cat. #AP8733c) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of SOD1 Antibody (Center) (Cat. #AP8733c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
SOD1 Antibody (Center) - Research Areas
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BACKGROUND
SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein.
REFERENCES
Crapo,J.D., et.al., Proc. Natl. Acad. Sci. U.S.A. 89 (21), 10405-10409 (1992)
