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CDC42EP3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CDC42EP3 Antibody (N-term) AP8775a
    Western blot analysis of CDC42EP3 Antibody (N-term) (Cat. #AP8775a) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.
    detail
  • WB - CDC42EP3 Antibody (N-term) AP8775a
    Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term) (Cat. #AP8775a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.
    detail
  • IHC-P - CDC42EP3 Antibody (N-term) AP8775a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CDC42EP3 Antibody (N-term) AP8775a
    CDC42EP3 Antibody (N-term) (Cat. #AP8775a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q9UKI2
Other Accession Q9CQC5, NP_006440
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27678 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 10602
Other Names Cdc42 effector protein 3, Binder of Rho GTPases 2, MSE55-related Cdc42-binding protein, CDC42EP3, BORG2, CEP3
Target/Specificity This CDC42EP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CDC42EP3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC42EP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC42EP3
Synonyms BORG2, CEP3
Function Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts.
Cellular Location Endomembrane system; Peripheral membrane protein. Cytoplasm, cytoskeleton
Tissue Location Highly expressed in the heart and weakly in the brain.
Research Areas
Citations (0)
citation

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Background

CDC42EP3 is probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. It induces pseudopodia formation in fibroblasts.

References

Simpson,J.C., et.al., EMBO Rep. 1 (3), 287-292 (2000)

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$ 182.50
$ 70.00
Cat# AP8775a
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