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CSGALNACT2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CSGALNACT2 Antibody (Center) AP8777C
    Anti-CSGALNACT2 Antibody (Center) at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CSGALNACT2 Antibody (Center) AP8777C
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CSGALNACT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CSGALNACT2 Antibody (Center) AP8777C
    CSGALNACT2 Antibody (Center) (Cat. #AP8777c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q8N6G5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 62572 Da
Antigen Region 200-229 aa
Additional Information
Gene ID 55454
Other Names Chondroitin sulfate N-acetylgalactosaminyltransferase 2, Chondroitin beta-1, 4-N-acetylgalactosaminyltransferase 2, Beta4GalNAcT-2, GalNAcT-2, CSGALNACT2, CHGN2, GALNACT2
Target/Specificity This CSGALNACT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from the Central region of human CSGALNACT2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCSGALNACT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CSGALNACT2
Synonyms CHGN2, GALNACT2
Function Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains.
Cellular Location Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein
Tissue Location Ubiquitous.
Citations ( 0 )

Background

Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). It is required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains.

References

Yu Y., et.al., Genome Res. 11:1392-1403(2001).

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$ 182.50
$ 70.00
Cat# AP8777C
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