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CD24 Antibody (N-Term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - CD24 Antibody (N-Term) AP8782a
    CD24 Antibody (N-Term) (Cat.# AP8782a) western blot analysis in Raji,Ramos cell line and rat cerebellum tissue lysates (35ug/lane).This demonstrates the CD24 antibody detected the CD24 protein (arrow).
  • WB - CD24 Antibody (N-Term) AP8782a
    Western blot analysis of CD24 Antibody (N-Term) (Cat. #AP8782a) in MCF-7 cell line lysates (35ug/lane). CD24 (arrow) was detected using the purified Pab.
  • IHC-P - CD24 Antibody (N-Term) AP8782a
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CD24 Antibody (N-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CD24 Antibody (N-Term) AP8782a
    CD24 Antibody (N-Term) (Cat.#AP8782a) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25063
Other Accession P24807, NP_037362
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 8097 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 100133941
Other Names Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen, CD24, CD24, CD24A
Target/Specificity This CD24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CD24.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD24 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD24
Synonyms CD24A
Function Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
Research Areas
Citations (0)

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CD24 is a sialoglycoprotein that is expressed on mature granulocytes and in many B cells. This protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface.


Kay,R.,, J. Immunol. 147 (4), 1412-1416 (1991)

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Cat# AP8782a
(40 western blots)
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