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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CYP8B1 Antibody (C-term)   

CYP8B1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CYP8B1 Antibody (C-term) AP8787B
    Western blot analysis of CYP8B1 Antibody (C-term) (Cat. #AP8787b) in K562 cell line lysates (35ug/lane). CYP8B1 (arrow) was detected using the purified Pab.
  • IHC-P - CYP8B1 Antibody (C-term) AP8787B
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CYP8B1 Antibody (C-term) AP8787B
    CYP8B1 Antibody (C-term) (Cat. #AP8787b) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UNU6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58068 Da
Antigen Region 471-501 aa
Additional Information
Gene ID 1582
Other Names 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase, CYP8B1, CYP12
Target/Specificity This CYP8B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-501 amino acids from the C-terminal region of human CYP8B1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP8B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP8B1
Synonyms CYP12
Function Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein
Tissue Location Liver.
Research Areas
Citations (0)

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CYP8B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless.


Zhang,M., J. Biol. Chem. 276 (45), 41690-41699 (2001)
Wang,J.,, Histochem. Cell Biol. 123 (4-5), 441-446 (2005)

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Cat# AP8787B
(40 western blots)
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