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BIN3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BIN3 Antibody (N-term) AP8794A
    Western blot analysis of BIN3 Antibody (N-term) (Cat. #AP8794a) in HepG2, K562 cell line lysates (35ug/lane). BIN3 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - BIN3 Antibody (N-term) AP8794A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with BIN3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NQY0
Other Accession Q5PPZ5
Reactivity Human
Predicted Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29665 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 55909
Other Names Bridging integrator 3, BIN3
Target/Specificity This BIN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BIN3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBIN3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BIN3
Function Involved in cytokinesis and septation where it has a role in the localization of F-actin.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Ubiquitously expressed except in brain.
Research Areas
Citations (0)
citation

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Background

BIN3 is a member of the BAR domain protein family. The encoded protein is comprised solely of a BAR domain which is predicted to form coiled-coil structures and proposed to mediate dimerization, sense and induce membrane curvature, and bind small GTPases. BAR domain proteins have been implicated in endocytosis, intracellular transport, and a diverse set of other processes.

References

Ren,G., et.al., Microbiol. Mol. Biol. Rev. 70 (1), 37-120 (2006)

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$ 182.50
$ 70.00
Cat# AP8794A
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