COTL1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, IHC-P, E |
---|---|
Primary Accession | Q14019 |
Other Accession | Q2HJ57, NP_066972 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 15945 Da |
Antigen Region | 115-142 aa |
Gene ID | 23406 |
---|---|
Other Names | Coactosin-like protein, COTL1, CLP |
Target/Specificity | This COTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-142 amino acids from the C-terminal region of human COTL1. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | COTL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | COTL1 |
---|---|
Synonyms | CLP |
Function | Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis. |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus |
Tissue Location | Widely expressed with highest levels in placenta, lung, kidney and peripheral blood leukocytes and lower levels in brain, liver and pancreas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.
References
Provost,P., et.al., Proc. Natl. Acad. Sci. U.S.A. 96 (5), 1881-1885 (1999)
Provost,P., et.al., J. Biol. Chem. 276 (19), 16520-16527 (2001)
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