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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PISD Antibody (Center)   

PISD Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PISD Antibody (Center) AP8829c
    Western blot analysis of PISD Antibody (Center) (Cat. #AP8829c) in mouse cerebellum tissue and mouse NIH-3T3 cell line lysates (35ug/lane). PISD (arrow) was detected using the purified Pab.
  • IHC-P - PISD Antibody (Center) AP8829c
    Formalin-fixed and paraffin-embedded human brain reacted with PISD Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PISD Antibody (Center) AP8829c
    PISD Antibody (Center) (Cat. #AP8829c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9UG56
Other Accession NP_055153
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46672 Da
Antigen Region 223-250 aa
Additional Information
Gene ID 23761
Other Names Phosphatidylserine decarboxylase proenzyme, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, PISD
Target/Specificity This PISD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the Central region of human PISD.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPISD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PISD
Cellular Location Mitochondrion.
Research Areas
Citations (0)

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Background

Phosphatidylserine decarboxylases catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits

References

Simpson,J.C., et.al., EMBO Rep. 1 (3), 287-292 (2000)

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Cat# AP8829c
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(40 western blots)
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