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DOLK Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DOLK Antibody (Center) AP8834c
    Western blot analysis of DOLK Antibody (Center) (Cat. #AP8834c) in 293 cell line lysates (35ug/lane). DOLK (arrow) was detected using the purified Pab.
    detail
  • IHC-P - DOLK Antibody (Center) AP8834c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - DOLK Antibody (Center) AP8834c
    DOLK Antibody (Center) (Cat. #AP8834c) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9UPQ8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 59268 Da
Antigen Region 312-339 aa
Additional Information
Gene ID 22845
Other Names Dolichol kinase, Transmembrane protein 15, DOLK, KIAA1094, TMEM15
Target/Specificity This DOLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-339 amino acids from the Central region of human DOLK.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDOLK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DOLK
Synonyms KIAA1094, TMEM15
Function Catalyzes CTP-mediated phosphorylation of dolichol, the terminal step in de novo dolichyl monophosphate (Dol-P) biosynthesis (PubMed:12213788, PubMed:16923818, PubMed:17273964). Dol-P is a lipid carrier essential for the synthesis of N-linked and O-linked oligosaccharides and for GPI anchors (PubMed:12213788).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Ubiquitous.
Citations (0)
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Background

DOLK is involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors (By similarity).

References

Shridas,P. et.al., J. Biol. Chem. 281 (42), 31696-31704 (2006)

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$ 365.00
$ 140.00
Cat# AP8834c
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