DOLK Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | Q9UPQ8 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 59268 Da |
Antigen Region | 312-339 aa |
Gene ID | 22845 |
---|---|
Other Names | Dolichol kinase, Transmembrane protein 15, DOLK, KIAA1094, TMEM15 |
Target/Specificity | This DOLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-339 amino acids from the Central region of human DOLK. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DOLK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DOLK |
---|---|
Synonyms | KIAA1094, TMEM15 |
Function | Catalyzes CTP-mediated phosphorylation of dolichol, the terminal step in de novo dolichyl monophosphate (Dol-P) biosynthesis (PubMed:12213788, PubMed:16923818, PubMed:17273964). Dol-P is a lipid carrier essential for the synthesis of N-linked and O-linked oligosaccharides and for GPI anchors (PubMed:12213788). |
Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
Tissue Location | Ubiquitous. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DOLK is involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors (By similarity).
References
Shridas,P. et.al., J. Biol. Chem. 281 (42), 31696-31704 (2006)
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