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RAC2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RAC2 Antibody (C-term) AP8839b
    Western blot analysis of RAC2 Antibody (C-term) (Cat. #AP8839b) in K562 cell line lysates (35ug/lane). RAC2 (arrow) was detected using the purified Pab.
    detail
  • FC - RAC2 Antibody (C-term) AP8839b
    RAC2 Antibody (C-term) (Cat. #AP8839b) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P15153
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 21429 Da
Antigen Region 164-188 aa
Additional Information
Gene ID 5880
Other Names Ras-related C3 botulinum toxin substrate 2, GX, Small G protein, p21-Rac2, RAC2
Target/Specificity This RAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-188 amino acids from the C-terminal region of human RAC2.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAC2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAC2
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
Cellular Location Cytoplasm. Note=Membrane-associated when activated
Tissue Location Hematopoietic specific.
Research Areas
Citations (0)
citation

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Background

RAC2 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.

References

Reibel,L., et.al., Biochem. Biophys. Res. Commun. 175 (2), 451-458 (1991)
Brechard,S., et.al., J. Leukoc. Biol. 85 (4), 638-647 (2009)

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$ 182.50
$ 70.00
Cat# AP8839b
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