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MAG Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MAG Antibody (Center) AP8845c
    Western blot analysis of MAG Antibody (Center) (Cat. #AP8845c) in A375 cell line lysates (35ug/lane). MAG (arrow) was detected using the purified Pab.
  • FC - MAG Antibody (Center) AP8845c
    MAG Antibody (Center) (Cat. #AP8845c) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P20916
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 69069 Da
Antigen Region 439-466 aa
Additional Information
Gene ID 4099
Other Names Myelin-associated glycoprotein, Siglec-4a, MAG, GMA
Target/Specificity This MAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-466 amino acids from the Central region of human MAG.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAG Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAG
Synonyms GMA
Function Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Background

MAG is a type I membrane protein and member of the immunoglobulin uperfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions.

References

Stalder,A.K.,et.al.,J. Neuropathol. Exp. Neurol. 68 (2), 148-158 (2009)

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Cat# AP8845c
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(40 western blots)
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