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DTX4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DTX4 Antibody (N-term) AP8872a
    Western blot analysis of DTX4 Antibody (N-term) (Cat. #AP8872a) in HL-60 cell line lysates (35ug/lane). DTX4 (arrow) was detected using the purified Pab.
  • IHC-P - DTX4 Antibody (N-term) AP8872a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with DTX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - DTX4 Antibody (N-term) AP8872a
    DTX4 Antibody (N-term) (Cat. #AP8872a) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y2E6
Other Accession Q6PDK8
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67258 Da
Antigen Region 56-83 aa
Additional Information
Gene ID 23220
Other Names E3 ubiquitin-protein ligase DTX4, 632-, Protein deltex-4, Deltex4, RING finger protein 155, DTX4, KIAA0937, RNF155
Target/Specificity This DTX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human DTX4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDTX4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DTX4
Synonyms KIAA0937, RNF155
Function Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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DTX4 is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations


Storck,S.,, Mol. Cell. Biol. 25 (4), 1437-1445 (2005)

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Cat# AP8872a
(40 western blots)
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