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ALOX12 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • IF - ALOX12 Antibody (C-term) AP8877B
    Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term) (Cat. #AP8877b). AP8877b was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
  • WB - ALOX12 Antibody (C-term) AP8877B
    Western blot analysis of ALOX12 Antibody (C-term) (Cat. #AP8877b) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.
  • IHC-P - ALOX12 Antibody (C-term) AP8877B
    Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ALOX12 Antibody (C-term) AP8877B
    ALOX12 Antibody (C-term) (Cat. #AP8877b) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18054
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 75694 Da
Antigen Region 618-650 aa
Additional Information
Gene ID 239
Other Names Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Lipoxin synthase 12-LO, 332-, Platelet-type lipoxygenase 12, ALOX12, 12LO, LOG12
Target/Specificity This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12.
Dilution IF~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALOX12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALOX12
Synonyms 12LO, LOG12
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.
Cellular Location Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF
Tissue Location Expressed in vascular smooth muscle cells.
Research Areas
Citations (0)

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Oxygenase and 14,15-leukotriene A4 synthase activity.


Yoshimoto,T.,, Biochem. Biophys. Res. Commun. 172 (3), 1230-1235 (1990)

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Cat# AP8877B
(40 western blots)
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