|Application ||WB, IHC-P, IF, FC, E|
|Calculated MW||75694 Da|
|Antigen Region||618-650 aa|
|Other Names||Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Lipoxin synthase 12-LO, 332-, Platelet-type lipoxygenase 12, ALOX12, 12LO, LOG12|
|Target/Specificity||This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ALOX12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.|
|Cellular Location||Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF|
|Tissue Location||Expressed in vascular smooth muscle cells.|
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Provided below are standard protocols that you may find useful for product applications.
Oxygenase and 14,15-leukotriene A4 synthase activity.
Yoshimoto,T., et.al., Biochem. Biophys. Res. Commun. 172 (3), 1230-1235 (1990)
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