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ALOX12 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP8877b 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP8877b-ev20 20 ug 100 ul In Stock $ 95.00 Add to cart
  • Specification
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  • Reviews
  • Protocols
  • Backgrounds

ALOX12 Antibody (C-term) - Product info

ApplicationWB, IHC, IF, FC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP18054
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW75694 Da

ALOX12 Antibody (C-term) - Additional info

Gene ID 239
Other Names
ALOX12; LOG12; Arachidonate 12-lipoxygenase, 12S-type; Platelet-type lipoxygenase 12
Target/Specificity
This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627~656 amino acids from the C-terminal region of human ALOX12 .
Dilution
WB~~1:100~500
IHC~~1:10~50
IF~~1:10~50
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ALOX12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

ALOX12 Antibody (C-term) - Protein Information

Name ALOX12
Synonyms LOG12
Function
Oxygenase and 14,15-leukotriene A4 synthase activity.
Cellular Location
Cytoplasm.

ALOX12 Antibody (C-term) - Related products

AP8877b: ALOX12 Antibody (C-term)

RI10253: ALOX12 predesign siRNA

BP8877a: ALOX12 Antibody (N-term) Blocking Peptide

BP8877b: ALOX12 Antibody (C-term) Blocking Peptide

ALOX12 Antibody (C-term) - Application data

  • Western blot analysis of ALOX12 Antibody (C-term) (Cat. #AP8877b) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Confocal immunofluorescent analysis of ALOX12 Antibody (C-term) (Cat. #AP8877b) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • ALOX12 Antibody (C-term) (Cat. #AP8877b) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ALOX12 Antibody (C-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Oxygenase and 14,15-leukotriene A4 synthase activity.

REFERENCES

Yoshimoto,T., et.al., Biochem. Biophys. Res. Commun. 172 (3), 1230-1235 (1990)