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H3-S10 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP8890a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP8890a-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
H3-S10 Antibody - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P84243 |
| Other Accession | P61830, P02299, P08898, P02302, P02301, Q6NXT2, A5PK61, Q6PI79, P84245, P84246, Q71LE2, P84244, P84249, Q6PI20, P84247, Q5E9F8, Q27532, Q9U281, Q10453, P84233, P84228, Q71DI3, Q4QRF4, P84229, P84227, Q6LED0, P68433, P68431, P68432, Q16695 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Drosophila, C.Elegans, Mouse, Pig, Rat, Rabbit, Xenopus, Yeast, Zebrafish |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 15328 Da |
H3-S10 Antibody - Additional info | |
| Gene ID 3021 | |
| Other Names H3F3A; H3.3A; H3F3; Histone H3.3 | |
| Target/Specificity This H3-S10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human H3-S10. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions H3-S10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
H3-S10 Antibody - Protein Information | |
| Name H3F3A | |
| Synonyms H3.3A, H3F3 | |
| Function Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
H3-S10 Antibody - Related products
AM1151a: Phospho-Histone H3-S10 Antibody
AM1151b: Phospho-Histone H3-S10 Antibody
AM1151c: Phospho-Histone H3-S10 Antibody
AP1050a: Histone H3-MeK9 Antibody
AP1051d: Histone H3 Antibody (S10)
AP3667a: Phospho-H3-S10 Antibody
AP9934a: Histone H3-K9 Antibody
RI12390: H3F3B predesign siRNA
DC00525: Human H3F3B cDNA Clone
LY11083a: H3F3B Over-expression Lysate
BP1050a: Histone H3-MeK9 Antibody Blocking Peptide
BP1051a: Histone H3 (N-term) Blocking Peptide
BP1051d: Histone H3 Antibody (S10) Blocking Peptide
BP3667a: Phospho-H3-S10 Antibody Blocking Peptide
BP8890a: H3-S10 Antibody Blocking Peptide
BP9934a: Histone H3-K9 Antibody Blocking Peptide
H3-S10 Antibody - Application data
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Western blot analysis of Phospho-H3-S10.ctrl (Cat. #AP8890a) in CEM cell line lysates (35ug/lane). H3 (arrow) was detected using the purified Pab.
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H3-S10 Antibody (RB21591) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H3-S10 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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H3-S10 Antibody (Cat. #AP8890a) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
H3-S10 Antibody - Research Areas
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BACKGROUND
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.
REFERENCES
Wells,D. et.al., Proc. Natl. Acad. Sci. U.S.A. 82 (9), 2834-2838 (1985)
