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H3-S10 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
United StatesOrdering Information
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|AP8890a||400 µl (40 western blots)||No||Call||DISCONTINUED INQUIRE CLICK INQUIRE Add to cart|
|AP8890a-ev||80 µl (8 western blots)||In Stock||Call||DISCONTINED INQUIRE CLICK INQUIRE Add to cart|
- Citations : 0
H3-S10 Antibody - Product info
|Application||WB, IHC, FC|
|Other Accession||P61830, P02299, P08898, P02302, P02301, Q6NXT2, A5PK61, Q6PI79, P84245, P84246, Q71LE2, P84244, P84249, Q6PI20, P84247, Q5E9F8, Q27532, Q9U281, Q10453, P84233, P84228, Q71DI3, Q4QRF4, P84229, P84227, Q6LED0, P68433, P68431, P68432, Q16695|
|Predicted||Bovine, Chicken, Drosophila, C.Elegans, Mouse, Pig, Rat, Rabbit, Xenopus, Yeast, Zebrafish|
|Calculated MW||15328 Da|
H3-S10 Antibody - Additional info
|Gene ID 3021|
H3F3A; H3.3A; H3F3; Histone H3.3
This H3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human H3.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
H3-S10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
H3-S10 Antibody - Protein Information
|Synonyms H3.3A, H3F3|
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
H3-S10 Antibody - Application data
Western blot analysis of Phospho-H3-S10.ctrl (Cat. #AP8890a) in CEM cell line lysates (35ug/lane). H3 (arrow) was detected using the purified Pab.
H3-S10 Antibody (RB21591) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H3-S10 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
H3-S10 Antibody (Cat. #AP8890a) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
H3-S10 Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.
Wells,D. et.al., Proc. Natl. Acad. Sci. U.S.A. 82 (9), 2834-2838 (1985)