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OAT Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - OAT Antibody (N-term) AP8897a
    All lanes : Anti-OAT Antibody (N-term) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - OAT Antibody (N-term) AP8897a
    OAT Antibody (N-term) (Cat. #AP8897a) western blot analysis in 293,293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane).This demonstrates the OAT antibody detected the OAT protein (arrow).
  • IF - OAT Antibody (N-term) AP8897a
    Fluorescent image of A549 cell stained with OAT Antibody (N-term)(Cat#AP8897a/SA100310AG).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with OAT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).OAT immunoreactivity is localized to Mitochondrion significantly.
  • IHC-P - OAT Antibody (N-term) AP8897a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - OAT Antibody (N-term) AP8897a
    OAT Antibody (N-term) (Cat. #AP8897a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04181
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48535 Da
Antigen Region 27-55 aa
Additional Information
Gene ID 4942
Other Names Ornithine aminotransferase, mitochondrial, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, Ornithine aminotransferase, hepatic form, Ornithine aminotransferase, renal form, OAT
Target/Specificity This OAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human OAT.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOAT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OAT
Cellular Location Mitochondrion matrix
Research Areas
Citations (0)

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OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.


Michaud J.,, Am. J. Hum. Genet. 56:616-622(1995).

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$ 295.00
$ 99.00
Cat# AP8897a
(40 western blots)
Availability: In Stock
Bulk Size
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