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EDF1 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP8927c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP8927c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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EDF1 Antibody (Center) - Product info | |
| Application | WB, IHC
|
| Primary Accession | O60869 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 16369 Da |
EDF1 Antibody (Center) - Additional info | |
| Gene ID 8721 | |
| Other Names EDF1; Endothelial differentiation-related factor 1; Multiprotein-bridging factor 1 | |
| Target/Specificity This EDF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~119 amino acids from the Center region of human EDF1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format PPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions EDF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
EDF1 Antibody (Center) - Protein Information | |
| Name EDF1 | |
| Function Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1 Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism | |
| Cellular Location Cytoplasm. Nucleus. Note=Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin | |
| Tissue Location Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues | |
EDF1 Antibody (Center) - Related products
AP8927c: EDF1 Antibody (Center)
DC04099: Human EDF1 cDNA Clone
EDF1 Antibody (Center) - Application data
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Western blot analysis of EDF1 Antibody (Center) (Cat. #AP8927c) in Y79 cell line lysates (35ug/lane). EDF1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with EDF1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
EDF1 Antibody (Center) - Research Areas
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BACKGROUND
EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.
REFERENCES
Humphray,S.J.,et.al., Nature 429 (6990), 369-374 (2004)
