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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CDC37 Antibody (Center)   

CDC37 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CDC37 Antibody (Center) AP8965C
    All lanes : Anti-CDC37 Antibody (Center) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CDC37 Antibody (Center) AP8965C
    Western blot analysis of CDC37 Antibody (Center) (Cat. #AP8965c) in MCF-7, A2058 cell line lysates (35ug/lane). CDC37 (arrow) was detected using the purified Pab.
  • IHC-P - CDC37 Antibody (Center) AP8965C
    CDC37 Antibody (Center) (Cat. #AP8965c) IHC analysis in formalin fixed and paraffin embedded skin followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDC37 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - CDC37 Antibody (Center) AP8965C
    CDC37 Antibody (Center) (Cat. #AP8965c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q16543
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44468 Da
Antigen Region 116-144 aa
Additional Information
Gene ID 11140
Other Names Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit, p50Cdc37, Hsp90 co-chaperone Cdc37, N-terminally processed, CDC37, CDC37A
Target/Specificity This CDC37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the Central region of human CDC37.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC37 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC37
Synonyms CDC37A
Function Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206).
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

CDC37 is a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.

References

Dai,K., et.al., J. Biol. Chem. 271 (36), 22030-22034 (1996)
Lamphere,L., et.al., Oncogene 14 (16), 1999-2004 (1997)

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$ 295.00
$ 99.00
Cat# AP8965C
Size:
Quantity:
(40 western blots)
Availability: In Stock
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